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The following plugins allow you to remove transparency or force alpha values to 255: Remove Transparency 1.0
In digital imaging, each pixel has four values: Red, Green, Blue, and (transparency). paint net alpha to 255 plugin download
If you want to quickly make a transparent layer fully opaque without a plugin: multiple times (Ctrl+Shift+D). Merge the layers down (Ctrl+M). The following plugins allow you to remove transparency
The fastest method is using the plugin.
The official, safe is hosted on the Paint.NET Forum Community Plugins section. As of the latest update, here is the direct path: each pixel has four values: Red